required for the same loci to be co-amplified in multiplex mixtures for the m-PCR in the test. Based on yield of PCR
products for the five target genes, the results showed an optimal multiplex annealing temperature of 54 C. Determination of detection sensitivity of the m-PCR The sensitivity of the m-PCR was appraised by evaluating the DNA extract mixture of the serial dilutions of the five target pathogens (Fig. 4). As seen, a detection limit of 103
cfu/mL was noted for Salmonella, E. coli O157:H7,