Introduction
Fluorescent dyes have the unique property of absorbing in the UV range and emitting in the visible range of the color spectrum. Each fluorescent dye has its own unique properties and emission spectrum of fluorescent dyes often vary with environmental conditions. Thus, many fluorescent dyes can be used as sensors to probe their local environments in biological and analytical applications. For example, coumarin [1], rhodamine [2], and oxazine [3] dyes are known to be efficiently quenched by the nucleobase guanosine (dG) and the amino acid tryptophan (Trp). In order to obtain an accurate indicator for fluorescent dyes, an accurate analysis of the color and fluorescence is necessary. Therefore, a system to measure the fluorescence spectra of high quality is needed. The current laser-induced fluorescent (LIF) system is one popular measurement system used