All the treatments were run in duplicate. The pH of the fresh chopped banana peel (80% moisture) was adjusted to pH 7 using 0.1-N hydrochloric acid (HCl) /0.1-N sodium hydroxide (NaOH) and sterilized in autoclave for 15 min at 121°C. After cooling, inoculum (5 mL) was added to each flask in the laminar chamber with the help of sterilized pipette. The flasks were then incubated at 35°C for 24 h without shaking in incubator.
The SSF media flasks were gently shaken after every 12 h for uniform mixing of the substrate and microorganism