In this study, we describe the combination of transmission surface plasmon resonance (TSPR) and
electrochemical techniques for the application to biosensors with conducting polymers. Electropolymerization was employed
to construct poly(pyrrole-3-carboxylic acid) (PP3C) film on a gold-coated grating substrate using pyrrole-3-carboxylic acid
(P3C) monomer solution in 0.5 M H2SO4. In situ electrochemical-transmission surface plasmon resonance (EC-TSPR)
measurements were carried out to study the kinetic and electroactivity properties of PP3C film. Immobilization of antihuman IgG
on the activated surface and the binding process of human IgG and antihuman IgG in neutral solution could be detected in situ
by EC-TSPR measurement. The surface modification steps on the PP3C layer led to an increase in intensity of the transmission
peak. The performance, sensitivity, and utility of EC-TSPR spectroscopy showed obvious advantages for the detection of binding
process with the simple experimental setup, and could be applied to the study of biomolecular interactions in various systems.