Myosin heavy chain (MHC) isoform separation and Western blots of human,
caracal and lion skeletal muscles. A rat sample was included in 3E as a positive control
for MHC IIb. A. Silver stain (arrow indicates band that was not recognizable by any of
the primary antibodies used). B. MHC IIa (antibody 2F7). C. MHC IIx (antibody 6H1).
D. MHC I and MHC IIx (antibodies BA–D5 and 6H1). E. MHC IIb (antibody BF–F3).