When comparing the biological efficiency of mushroom production, the highest yield of fruiting bodies was obtained using a mixture of date waste and rice straw at a ratio (1:1) (11.96%), followed by a mixture 3:1 (11.16%). The lowest one was the mixture 2:1 (9.19%). FungusPleurotus ostreatus NRRL-0366 can also be cultivated on mango waste supplemented with rice straw at a different ratio.