where t and t sstart and finish times Žh. of measurement period; C sconcentration 0 1 t
of oxygen in the water Žml O ly1 . at time t; V s volume of respirometer ŽL.; 2 r
V svolume of the mussels ŽL.. m
The ammonia analysis was carried out by collecting water samples from the
respiration chambers, and from the experimental control without mussels. Three replicates
of water samples Ž10 ml per sample. were taken and analyzed using a phenol-hypochlorite
method ŽSolo´rzano, 1969.. The ammonia excretion rate was determined by
subtracting ammonia concentrations in the control chamber over treatment chambers.