Impact of age on the length and complexity of glial processes
in the stratum radiatum. Astroglial process numbers and length were
drawn on a printed image, scaled up and analyzed with NIH software
ImageJ running the Sholl Analysis Plugin v1.0. As shown by the
scheme over the graph, a grid with concentric rings or shells
distributed at equal distances d centered on the soma of a cell was
superimposed on GFAP immunoreactive astrocyte images. The
number of process intersections i per shell was computed and
branching complexity evaluated. The length of the processes was
estimated by the sum of the products of d by i for each ring. Holm–
Sidak post hoc test; Double asterisks represent highly significant
differences (P