Digestions conducted in the RVA gave significantly higher vis- cosities and solubility values than the same treatments digested in the shaking water bath (p < 0.05). The paddle, which fills a large proportion of the canister volume, mixed the slurries efficiently. In the shaking water bath, the bread imbibed most of the buffer and the marbles were not able to move freely in the centrifuge tube. The improved mixing in the RVA would account for the increased extraction and viscosity. When digestion was conducted in the RVA, the resulting extract viscosity was approximately double the viscosity measured after digestion in the shaking water bath (Table 1). However, the extract viscosities measured using both methods were highly correlated (R2 ¼ 0.92) in agreement with a previous
study (Gamel et al., 2012). This suggests that the simplified method could be a suitable alternative to the more labor intensive water bath method.