Betaine has been well-established as a labile methyl donor and as an osmoprotectant, which is synthesized via the oxidation of betaine aldehyde formed by the irreversible oxidation of choline in the mitochondria of the liver and renal tissues (Ratriyanto et al., 2009). Betaine is added to the feed of laying hens at a level of 600 ppm to significantly improve laying performance. So, it consequently contributes to enhanced productivity which could have an important economic value (Park et al., 2006). The addition of betaine to the feeds in particular improves performance under stress conditions that affect cell osmolarity. Furthermore, betaine promotes intestinal microbes against osmotic variations and improves microbial fermentation activity, which in turn, may enhance nutrient digestibility (Ratriyanto et al., 2009).