In the present paper, a pathogenic V. cholerae strain was isolated from vibriosis-infected freshwater-farmed P. vannamei, which was characterized by leg yellowing, in Hangzhou, Zhejiang, China. Phenotypic characterization, taxonomic position, virulence genes and control of the strain with the predatory bacterium Bdellovibrio bacteriovorus were examined. To the best of our knowledge, this is the first report of the control of the shrimp pathogen V.cholerae with B. bacteriovorus