So when ovulation was observed, the eggs were stripped manually after the latency period (mean time between injection and ovulation [23]) and weighed from each female separately, the weight being expressed as percentage of female BW. The number of eggs released was calculated following the gravimetric method [34]. Assessment of ovulation was carried out by determining the ovulation success (number of ovulated females/number of injected) 100% and by ovulation index (OI) weight