For nematode inoculum preparation, few plant roots
infected with root-knot nematode were collected from
greenhouses in Chaharmahal va Bakhtiari province and egg
masses on them cultured on the tomato plant variety Rut-
gers using single egg mass culture technique. After iden-
tification of nematode species, using eggs and larva, the
target nematode population was cultured on the same tomato plants. For making enough nematode inoculum,
eggs of M. javanica were extracted from the infected roots
using sodium hypochlorite (Hussey and Barker 1973).