Using gene expression profiling, Malone et al. (2008) isolated >30 genes which are up-regulated, and >30 which are downregulated, following exposure to high yet sub-lethal pressures.
Benito et al. (1999) found significant differences in pressure sensitivity (>4 log) of different E. coli O157:H7 isolates suspended in buffer at 500 MPa for 15 min, as well as differences in heat sensitivity and pH in combination with HPP.
While the basic virulence gene profile for the 39 isolates used in this study were provided by those donating them, genomic DNA sequence for most of the 39 STEC is currently unknown.
Genomic DNA sequencing, in combination with transcriptomics and proteomics, may elucidate which genes and proteins may be responsible for the >25 fold difference in resistance to high pressure