Twenty microliters of the reaction mixture was injected
onto an HPLC equipped with a fluorescence detector
(excitation 390 nm, emission 475 nm) and a 3-cm 3 3-μm
analytical co1umn. The mobile phase was acetonitrile : 1%
aqueous KCl : acetic acid (4059: 1). Quantitation was measured
as peak height relative to fumonisin B1 standards.