Due to the fast decrease observed in pollen viability at roomtemperature (see Section 3, Fig. 1), a method for short-termpollen storage was developed. Since avocado shows protogynousdichogamy, different pollen conservation mechanisms should beconsidered based on the floral groups of the parental genotypes.If the crosses are made using genotypes of complementary floralgroups, the pollen collection can be made just before performingthe hand pollinations although, with the purpose of optimizing thenumber of crosses during the effective period of pollination, pollencollection can be performed once a day, especially if the genotypesof interest for the crosses are far away from each other. Thus, theestablishment of a short-term pollen storage method under orchardconditions without reducing pollen viability will be useful.