The male buds were immersed in 70% Clorox containing of three drops Tween-20 for five minutes and
were rinsed with sterilized distilled water for three times. This procedure was repeated and after rinsing three
times by sterilized distilled water, they were immersed for three min in 70% ethanol. The male buds were
rinsed with sterilized distilled water for three times and this step was repeated. The male bud explants were
cut into two portions and cultured on MS media after rinsing by sterilized distilled water.