For the 150 mg Cu/kg amended soil, the highest copper accumulation in shoots of Brassica juncea (L.) Czern and Bidens alba (L.) DC. var radiata were 587 and 180 mg Cu/kg (in shoots dry matter), whereas in roots, the amount of copper accumulated were 3,184 and 699 mg Cu/kg (in roots dry matter), respectively. These results were very similar to the study performed by Morishima and Oka.