After pressure treatment,
spores were briefly sonicated (11 W, 3 s) to disrupt spore clumps
formed during the pressure treatment and then incubated at 40 C.
Germination was monitored by measuring the OD600 of the spore
suspension (Smartspec 3000 Spectrophotometer, Bio-Rad Laboratories,
Hercules, CA, USA). S
After pressure treatment,spores were briefly sonicated (11 W, 3 s) to disrupt spore clumpsformed during the pressure treatment and then incubated at 40 C.Germination was monitored by measuring the OD600 of the sporesuspension (Smartspec 3000 Spectrophotometer, Bio-Rad Laboratories,Hercules, CA, USA). S
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