2.7. Phytotoxicity studies
In order to assess the toxicity of untreated and treated dyes, phytotoxicity tests were performed (Saratale et al., 2009). The decolorization metabolites of Acid Red B extracted by ethyl acetate were dried and dissolved in sterile distilled water. Seeds of Phaselus mungo and Oryza sativa (ten seeds of each) were watered separately per day with 10 mL sample of Acid Red B solution (1000 mg/L) or its decolorization metabolism. Meanwhile, control set was executed using sterile distilled water. Both of germination and the lengths of plumule and radical were observed and recorded after a week