A chondrocyte progenitor population isolated from the surface zone of articular cartilage has become a promising cell source for
cell-based cartilage repair. The cartilage-derived stem/progenitor cells aremultipotent stem cells, which can differentiate into three
cell types in vitro including adipocytes, osteoblasts, and chondrocytes.Much work has been done on cartilage stem/progenitor cells
(CSPCs) from people, horses, and cattle, but the relatively little literature has been published about these cells in chickens. In our
work,CSPCswere isolated fromchicken embryos in incubated eggs for 20 days. In order to inquire into the biological characteristics
of chicken CSPCs, immunofluorescence, reverse transcription-polymerase chain reaction (RT-PCR), and flow cytometry were
adopted to detect the characteristic surface markers of CSPCs. Primary CSPCs were subcultured to passage 22 and, for purpose
of knowing the change of cell numbers, we drew the growth curves. Isolated CSPCs were induced to adipocytes, osteoblasts, and
chondrocytes. Our results suggest that we have identified and characterised a novel cartilage progenitor population resident in
chicken articular cartilage and CSPCs isolated from chickens possess similar biological characteristics to those from other species,
which will greatly benefit future cell-based cartilage repair therapies.
A chondrocyte progenitor population isolated from the surface zone of articular cartilage has become a promising cell source forcell-based cartilage repair. The cartilage-derived stem/progenitor cells aremultipotent stem cells, which can differentiate into threecell types in vitro including adipocytes, osteoblasts, and chondrocytes.Much work has been done on cartilage stem/progenitor cells(CSPCs) from people, horses, and cattle, but the relatively little literature has been published about these cells in chickens. In ourwork,CSPCswere isolated fromchicken embryos in incubated eggs for 20 days. In order to inquire into the biological characteristicsof chicken CSPCs, immunofluorescence, reverse transcription-polymerase chain reaction (RT-PCR), and flow cytometry wereadopted to detect the characteristic surface markers of CSPCs. Primary CSPCs were subcultured to passage 22 and, for purposeof knowing the change of cell numbers, we drew the growth curves. Isolated CSPCs were induced to adipocytes, osteoblasts, andchondrocytes. Our results suggest that we have identified and characterised a novel cartilage progenitor population resident inchicken articular cartilage and CSPCs isolated from chickens possess similar biological characteristics to those from other species,which will greatly benefit future cell-based cartilage repair therapies.
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