94°C) and ended by a 10-min extension step at 72°C. The PCR-amplified samples
were subjected to electrophoresis (90 min, 90 V) in a 2% agarose gel (FMC)
with 13 TAE buffer (40 mM Tris-acetate, 1 mM EDTA [pH 8]) and stained
with ethidium bromide after the run. The DNA molecular weight marker VI
(Boehringer Mannheim) was used to estimate the size of the amplified fragment.