Studies have shown that there is a significant difference
in the obtained LC50 results for different solvents’ extracts,
mainly because some solvents are a poor medium
for obtaining specific bioactive components (responsible
for the toxicity) from the plant sample, than others (Lalisan
et al., 2014).
Dimethylsulfoxide is widely used solvent for the reconstitution
of evaporated plant extracts, because brine
shrimp nauplii show no significant sensitivity to this solvent
up to 11% concentration (Ahmed et al., 2013; Musa,
2012; Kamba and Hassan, 2010). Preparation of stock solution
and several dilutions (suggested by McLaughlin,
1991) provide different dose levels, which gives opportunity
to determine the linear increase of the toxicity by increasing
the concentration of the toxic compound(s) present
in the plant extract.