The ultrasonic waves
with frequency 35 kHz applied for 10, 20 and 30 min led to obtain
half population of cells, which have larger cross-section area than
the value of 0.0040 mm2 (4007 lm2), 0.0162 mm2 (16,169 lm2)
and 0.0298 mm2 (29,851 lm2), respectively (Fig. 4b).