PLE, the meat product was applied on disposable glass-fibre filters, the temperature was set to 30 °C, and a static time of 15 min was applied. Furthermore after homogenisation in the ball mill 50 mg defatted and dehydrated material were used for the protein extraction performed with 450 μL TRIS-HCl (1 M, pH 8.2) and 50 μL of solvent A containing the internal standard (5 ng/μL each). The extraction time was reduced to 2 h.