A library was constructed on the combined V4 region of 16S rDNA
district based on Illumina Miseq technology. For the archaea, primer
pairs 5′-CAGYMGCCRCGGKAAHACC-3′ and 3′-GGACTACNSGGGTMTC
TAAT-5′ were used. For the bacteria, primer pairs 5′-GTGCCAGCMGC
CGCGGTAA-3′ and 3′-GGACTACHVGGGTWTCTAAT-5′ were employed.
The design of primers and sequencing were provided by Novogene
Co., Ltd. in Beijing