Two perpendicular diameters were drawn on the bottom of
the Petri-dishes (Plates) containing the various culture
media used for the study, so that they intercept at the
centre of the plates. In each case, the inoculum was placed
at the centre of the medium (i.e. loopful of active growing
mycelial per plate). This was evenly spread in a circular
manner within the centre of the medium (about 3mm
diameter).
The plates was incubated at 300C and observed for nine
(9) days during which the mycelial vegetative growth and
mycelial density of pleurotus pulmonarius were recorded.
The mycelial density was rated as described by Kadiri
(1998) as follows:
+ = Very Scanty
2+ = Scanty
3+ = Moderate
4+ = Abundant
5+ = Very abundant
The growth rate is given by the formular below:
Growth rate = Colony diameter on the last day(cm)
Number of days measurement was taken after inoculation
Daily mycelial growth was determined using a ruler across
the Petri-dish horizontally.