Recovery was routinely checked by addition of toxin standard solutions to degassed and pH-adjusted beer samples before further processing. For each test system, 3e4 beer samples were spiked with toxin concentration levels of 10e20 mg/L (DON), 0.5e2.0 mg/L (ZEA), 0.5e2.0 mg/L (ergometrine), and 1.0e2.0 mg/L (AOH), respectively. After an incubation step of 30 min, spiked samples were further processed according to 2.2. and tested subsequently. Calculated toxin contents in naturally contaminated beer samples were not corrected for the analytical recovery rates. assayed. In addition, the 50% inhibition concentration (IC50) values and the LODs of the standard curves were recorded, to check the stability of the EIAs over the period of analyses.
Recovery was routinely checked by addition of toxin standard solutions to degassed and pH-adjusted beer samples before further processing. For each test system, 3e4 beer samples were spiked with toxin concentration levels of 10e20 mg/L (DON), 0.5e2.0 mg/L (ZEA), 0.5e2.0 mg/L (ergometrine), and 1.0e2.0 mg/L (AOH), respectively. After an incubation step of 30 min, spiked samples were further processed according to 2.2. and tested subsequently. Calculated toxin contents in naturally contaminated beer samples were not corrected for the analytical recovery rates. assayed. In addition, the 50% inhibition concentration (IC50) values and the LODs of the standard curves were recorded, to check the stability of the EIAs over the period of analyses.
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