Non-defatted seed powders were used in this study. Total phenolic
contents of all extracts were analyzed using Folin–Ciocalteu
reagent. Methanol/water seed extracts (0.5 mL having 1 mg dry
extract) was mixed with sodium carbonate (2 mL, 7.5%) and Folin–Ciocalteu reagent (2.5 mL, 10%). The mixture was incubated
for 30 min at room temperature, then absorbance was recorded at
765 nm using a UV–VIS spectrophotometer (IRMECO, Geesthacht/
Germany, Model 5000). Total phenolic contents were quantified
based from absorbance measurements (Abs) and standard curve
for gallic acid (2–200 ppm) using the relations below;
Total phenols ðmg GAE=g sampleÞ ¼ Abs
m Vex DF 1
W
where, m (l/mg) = slope from the calibration graph, DF = Dilution
factor for sample before assay (1 if undiluted), Vex = Original volume
of sample extract and W = dry weight of seed extract (g).
2.