Intestinal samples were collected and fresh digesta
samples from ileum were taken for bacterial analyses within
1 h from collection. Digesta samples were serially diluted in
0.85% sterile saline solution for enumeration of Lactobacilli
spp. and Escherichia coli (E. coli) by conventional
microbiological techniques using selective agar media. All
microbiological analyses were performed in duplicate and
the average values were used for statistical analysis.
Lactobacilli spp. were anaerobically assayed using