Directions
Suspend 23 grams in 990 ml distilled water. Add 10 ml of Tributyrin (FD081). Mix and heat to boiling to dissolve the medium
completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Shake the flask and individual plate so as to
maintain uniform turbidity.
Note : For proper lipase activity, it is recommended to use glass plates instead of disposable plates. Hence USE ONLY GLASS
PLATES. DO NOT USE PLASTIC PLATES.