Total phenolics. A portion of 300 L from each sample was
diluted with 4.3 mL distilled water and 100 L Folin-Ciocalteu
reagent were added. After an interval of 3 min, 2% Na2CO3 was
added to 300 L portions, and the mixture vortexed and incubated
for 30 min. Absorbances were then read on a UV–vis (PerkinElmer,
Lambda–1050 spectrophotomer, CA, USA) spectrophotomer at
760 nm. Gallic acid was used as the standard. The results were
expressed as mg gallic acid equivalents (GAE)/g flesh weight.
2