The same number of animals were used for control group, and were
exposed to the same amount of distilled water. The
snails remained exposed for 24 hours and after this,
were transferred to similar terrariums containing 50
g of moistened sterilized mulch (120°C for 1 hour)
to remain more 24 h to recovery. During the 48 h
period of the assays snails were not fed and at the
end of recovery period the mortality was verified.