In our inhibition studies, ddPCR showed a clear advantage over qPCR in SDS-inhibited samples, while its tolerance to other tested inhibitors was comparable with qPCR. Significantly, the qPCR assays demonstrated more asymmetrical amplification/inhibition with EDTA as inhibitor, with unequal inhibition in reference and transgene reactions, while inhibition was more symmetrical on the ddPCR platform.