. A 400-lL aliquot of the sample was initially prepared and processed according to the method described by Gião et al. (2008), with a DNA (calf thymus DNA, from Sigma–Aldrich) solution of 0.25 mg/mL.
. A400-lL aliquot of the sample was initially prepared and processedaccording to the method described by Gião et al. (2008), with aDNA (calf thymus DNA, from Sigma–Aldrich) solution of0.25 mg/mL.