Isolated apical and axillary buds (Figure 1 B)
were placed on Murashige and Skoog (MS)
liquid medium (Murashige and Skoog, 1962).
The pH of the media was adjusted to 5.8 before
autoclaving at 121°C and 1.1 kg cm»2 pressure
for 15 min. Ten mililiters of liquid culture medium
were added per culture tube (25 x150 mm) with
paper bridges to avoid total inmersion of the
buds and plugged with nonabsorbent cotton
wrapped in cheese cloth. All cultures were
incubated in sun light growth rooms at 26 ± 2ºC.
Shoots were subcultured after 4 weeks