Then, 0.1 ml of heat-activated spores (to the final concentration of OD600w1.0) were cooled in water bath at ambient temperature for 5 min before mixing with 0.5 ml of the pre-warmed germinant equilibrated at 40 C or at room temperature (RT) (20 2 C).
Then, 0.1 ml of heat-activated spores (to the final concentration ofOD600w1.0) were cooled in water bath at ambient temperature for 5 min before mixing with 0.5 ml of the pre-warmed germinantequilibrated at 40 C or at room temperature (RT) (20 2 C).