A method for determination of metrafenone residues in bitter gourd and soil was developed. All samples
were extracted with ethyl acetate, purified with the glass column of florisil and NH2-SPE column, analyzed
by gas chromatography with electronic capture detector (GC-ECD). The results showed that it
had good linearity in the range of 0.01–2 mg/L and the correlation coefficient (r) was 0.9999. The average
recoveries of metrafenone in bitter gourd and soil were 83.51–91.75% and 84.76–91.72% with the relative
standard deviation of 3.48–9.18% and 4.23–7.25%, respectively. The limit of detection was estimated to be
0.005 mg/kg, the minimum concentration of detection in bitter gourd and soil was 1 102 mg/kg.