2. Materials and methods
2.1. Experimental design
Twenty glass tanks (90 cm 40 cm 35 cm) filled with 100 L
seawater were used as the experimental culture units. Temperature
in all tanks was maintained in the range of 27.3e28.3 C during the
entire experiment, aeration was provided in each aquarium using
an air blower and the light regime was set at 12 h light/12 h dark.
Inter-molt phase Pacific white shrimp juveniles, previously acclimatized
collectively to the experimental room and conditions for 1
week, at an initial average body weight of 2.02 ± 0.05 g were
randomly distributed in the tanks at a density of 30 shrimp/tank
(83 shrimp m2). Four times daily, a commercial pellet containing
30% of crude protein (Feng Li, PT Matahari Sakti, Indonesia) was
provided for 49 days to all tanks. The feeding level was determined
at 7% on wet body weight per day and the daily feed amount was
adjusted to the biomass in the tanks.