In the second photoperiod 12L/12D at 200C the gonadosomatic index was less in comparison to
19L/5D photoperiod. Male showed 4.6±0.6 and female showed 5.7±1.5 (fig-I). In this experimental
regime testis and ovary was in mature stage testis was creamy white in colour longer than right one
spermatogonia and primary spermatocytes were seen in histological preparations in female 6 out of 10
female fishes laid eggs. 2 male and 1 female were died and in 3 fishes egg laying was not seen. Ovary
was considerably white or yellowish in colour and oocytes were growing rapidly.(Fig-II b and III b).
In third experimental regime 8L/16D gonads were in immature stage.