Comparison of the RFLP patterns was the basis
of the assignment of markers to regions delineated by
the recombination breakpoints. RFLP probes abc156,
mwg68, ksuG9 and ksuE19 detected markers within
a region proximal to the rust resistance gene (region
ImpA; Fig. 1a, b), whereas the RFLP marker iag95
mapped distal to the stem rust resistance gene (region
ImpC). RFLP probe mwg60 identified markers within
the rye segment carrying SrR (region ImpB).