The enzymatic reduction of aldehydes by S. cerevisiae is
coupled to oxidation of the cofactors NADH or NADPH.
Therefore, both NADH and NADPH have to be continuously
regenerated. The major pathways which are involved
in regeneration are glycolysis and the pentose phosphate
pathway for NAD(H) and NADP(H), respectively [18].
Therefore, it may be expected that aldehyde reduction is
correlated with the glucose flux. Indeed, aldehyde reduction
was significantly decreased in the absence of glucose (Fig.
2). In addition, immobilized cells, which appeared to have a
higher glucose flux (Table 1), revealed a higher reduction
capacity than suspended cells.
The enzymatic reduction of aldehydes by S. cerevisiae iscoupled to oxidation of the cofactors NADH or NADPH.Therefore, both NADH and NADPH have to be continuouslyregenerated. The major pathways which are involvedin regeneration are glycolysis and the pentose phosphatepathway for NAD(H) and NADP(H), respectively [18].Therefore, it may be expected that aldehyde reduction iscorrelated with the glucose flux. Indeed, aldehyde reductionwas significantly decreased in the absence of glucose (Fig.2). In addition, immobilized cells, which appeared to have ahigher glucose flux (Table 1), revealed a higher reductioncapacity than suspended cells.
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