estimated in terms of the Trolox equivalent antioxidant capacity
(TEAC) in mg/mL Trolox/g.
2.2.4.4. Hydroxyl radical-scavenging activity assay. The hydroxyl
radical scavenging activity was determined according
to the method described by Liu, Wang, Xu, and Wang (2007)
with slight modifications. Briefly, 1 mL of the OLE (OFE/BPOL/
BPOF) solutions with different concentrations (0.2–1.2 mg/
mL) was added to 2 mL of sodium phosphate buffer (150 mM,
pH 7.4) containing 10 mM FeSO4, 2 mM sodium salicylate and
6mMH2O2.The mixture was incubated at 37 °C for 10 min, and
the absorbance of the solution was measured at 510 nm. The
hydroxyl radical-scavenging activity was calculated using the
equation 100 × (A0 − A1 + A2)/A0, where A0, A1 and A2 are the absorbances
of the control, samples, and samples without sodium
salicylate, respectively. Vc and BHT were used as positive
controls.