Bacterial cultures were grown on Nutrient agar (NA; Difco, Le Pontde Claix, France) for routine use, and maintained in Nutrient Broth (NB; Difco) with 15% glycerolat –80°C for long-term storage. Fungal pathogen was grown on Potato Dextrose agar (PDA; Difco) and maintained on PDA slant cultures at 4°C in the refrigerator until use.