The flowers (250g) were subjected to hydrodistillation for 3 h using an all glass Clevenger-type apparatus, to extract essential oil, according to the method recommended by theEuropean Pharmacopoeia.
[14]
The extracted essential oil sample was dried over anhydroussodium sulphate and stored in sealed vial at 4°C before gas chromatography/massspectrometric (GC/MS) analysis.