Micropropagation of pseudobulb segmentsIn vitro grown pseudobulbs were cut 0.5 to 1.0 cm size using sterilized surgical blade and forceps under the laminar air flow cabinet (HYSC, Korea). Then the cuttings of upper and lower part were put into the culture vessel containing 0.8% (w/v) agar solidified MS based micropropagation media supplemented withsixteen different concentrations and combinations of PGRs.