Antioxidant activity and total phenolic content of tamarind seed coat extracts (TSCEs) were compared between the two
extracts using boiling-water (TSCE-W) and 70% ethanol (TSCE-E) for extraction. TSCE-W, consisting of the highest
phenolic content, possessed 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and anti-lipid peroxidation activities
much higher than TSCE-E and Trolox. Additionally, both TSCEs also exhibited superoxide anion and hydrogen peroxide
scavenging activities higher than Trolox and BHA. Anti-lipid peroxidation and cytotoxicity of TSCE-W were also studied in
human foreskin fibroblast CCD-1064Sk cells. Cytotoxic effect was not observed when exposed to TSCE-W up to 1 mg/mL
for 12-48 h. However, TSCE-W significantly attenuated lipid peroxidation in H2O2-damaged cells. HPLC analysis showed
the presence of (+)-catechin, (-)-epicatechin, and procyanidin B2 in TSCE-W, which could be responsible for antioxidant
and anti-lipid peroxidation activities. The results suggest that an inexpensive and simple boiling-water extraction of
TSCE-W may provide a valuable natural antioxidant source having anti-lipid peroxidation for health food additives,
nutraceuticals as well as cosmeceuticals.
Antioxidant activity and total phenolic content of tamarind seed coat extracts (TSCEs) were compared between the two
extracts using boiling-water (TSCE-W) and 70% ethanol (TSCE-E) for extraction. TSCE-W, consisting of the highest
phenolic content, possessed 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and anti-lipid peroxidation activities
much higher than TSCE-E and Trolox. Additionally, both TSCEs also exhibited superoxide anion and hydrogen peroxide
scavenging activities higher than Trolox and BHA. Anti-lipid peroxidation and cytotoxicity of TSCE-W were also studied in
human foreskin fibroblast CCD-1064Sk cells. Cytotoxic effect was not observed when exposed to TSCE-W up to 1 mg/mL
for 12-48 h. However, TSCE-W significantly attenuated lipid peroxidation in H2O2-damaged cells. HPLC analysis showed
the presence of (+)-catechin, (-)-epicatechin, and procyanidin B2 in TSCE-W, which could be responsible for antioxidant
and anti-lipid peroxidation activities. The results suggest that an inexpensive and simple boiling-water extraction of
TSCE-W may provide a valuable natural antioxidant source having anti-lipid peroxidation for health food additives,
nutraceuticals as well as cosmeceuticals.
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