2.4. Raw fish oil
The water/fat mixture removed from MDM was hydrothermally
processed by heating in a water bath at 40 °C for 3 h. When the oil
began to separate from the water/fat mixture, supernatant oil was
transferred to another beaker using filtration (Whatman 4, GE
Healthcare, Brazil). The remaining slurry was discarded (Menegazzo
et al., 2014). Oils were prepared in duplicate from two independent
MDM lots, totaling four aliquots (eight considering
both fish oils). Each aliquot was divided into 13 smaller aliquots
(samples) of 30 mL each, stored in 50 mL amber glass bottles
(Duran Group GmbH, Mainz, Germany) in the absence of light at
18 °C for characterization. Each sample corresponded to a time
of analysis, each 15 days, from day 0 to day 180.