Sectioning of the mesocardiac, zygocardiac, and pterocardiac ossicles
revealed that when marking was successful, calcein was generally
incorporated into these structures at the edge of the endocuticle and
non-calcified membranous layer (growing edge) following immersion.
In individuals sacrificed more than 72 h postmolt, and when calcein
was present, it was generally deposited along the epicuticle (base) of
the new ossicles. Calcein was not present in the new ossicles of any individuals
sacrificed less than 72 h postmolt. In one individual sacrificed
eight weeks postmolt, calcein was present within the ossicles, between
the exocuticle and endocuticle (Fig. 2c). The calcein appears as a distinct
line throughout the structures and is not diffused across the structures,
indicating the rapid resorption of calcein at a time following molting,
and its incorporation into the new ossicles.