Immunization
Nine cross breed calves (10–12 month old) were treated with
Albendazole [Albomol] at 7.5 mg/kg body weight orally
one month prior to immunization. For immunization, animals
were divided randomly into three groups comprising of
three animals in each group. Groups 1 immunized with
rHaa86 and group 2 and 3 was kept as adjuvant and negative
control (inoculated with PBS only), respectively. The frozen
rHaa86 protein samples (100 lg/ml) were thawed and
emulsified thoroughly with equal volume of adjuvant (10 %
Montanide 888 in mineral oil). Animals of group 1 was
inoculated with 2 ml of reconstituted rHaa86 vaccine on 0,
30 and 60th day. The immunization was carried out by deep
intramuscular inoculation in the glutial muscle.